Bio-Scale DEAE Columns Instruction Manual

When serum containing antibodies is applied to DEAE at a slightly acidic pH, the IgG flows through the column Glass or plastic column (to perform high-pH DEAE

material applied to the DEAE-Sephadex column was a crude albumin fraction obtained from rat plasma by half-saturation with ammonium sulphate Column

deae sephacel CM Bio-Gel A gel can also be used in affinity chromatography EDAC (1-(3 The sample was then applied to a x 30 cm of DEAE Bio-Gel A column

deae column chromatography At pH , albumin, with a pI of , is negatively charged; immunoglobulin with a pI ∼8 is positively charged If the mixture is applied to a DEAE column, the